Anti-Dopamine beta Hydroxylase Polyclonal Antibody
| Host / Isotype: |
Sheep |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) Bovine (Bv) |
| Immunogen: |
Synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. |
|
| Immunocytochemistry (ICC) |
1:2,500 |
| Immunofluorescence (IF) |
1:5,000 |
| Immunohistochemistry (Frozen) (IHC (F)) |
Assay dependent |
| Western Blot (WB) |
1:5,000 |
| Pierce Anti-Dopamine beta Hydroxylase Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
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| PA3-925 |
100 µl |
$354.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 ul of antiserum containing 0.05% sodium azide. |
PA3-925 detects human, bovine, rat and mouse DBH. This antibody is not effective for immunohistochemistry in human brain.
PA3-925 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~72 kDa protein representing DBH from rat neurons. In immunohistochemistry and immunofluorescence procedures, it specifically stains chromaffin cells of the adrenal medulla, and DBH containing cells of the sympathetic nervous system.
The PA3-925 immunogen is a synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. This sequence is highly conserved (>85% homology) in human, bovine and mouse.
Figure 1 shows an immunohistochemistry stain of DBH in rat neuron tissue using PA3-925.
Figure 2 shows an immunofluorescence staining of DBH in rat neuron cells using PA3-925. |
DBH (Dopamine-Beta-hydroxylase) is an oxidoreductase belonging to the copper Type II, ascorbate dependent monooxygenase family. DBH is the enzyme responsible for conversion of dopamine to noradrenaline. Tissue specificity consists of chromaffin granules of the adrenal medulla and synaptic vesicles of the sympatheic nervous system. It exists as both a soluble and membrane-bound form in chromaffin granules. |
 
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This product is for In Vitro experimental use only
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