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Anti-PDX-1 Polyclonal Antibody

Printable Datasheet

Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Clone:
Label:
Species Reactivity: Human (Hu) Murine (Ms)
Immunogen: Synthetic Peptide: Q(21) R G P A P E F S A S P P A C(35)
Applications Dilution
Immunofluorescence (IF) 1:500
Immunohistochemistry (IHC) 1:500
Western Blot (WB) 1:3,000
Ordering Information
Pierce Anti-PDX-1 Polyclonal Antibody
Product Number Pkg Size Price


PA3-830 100 µl $354.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ul of antiserum containing 0.05% sodium azide.


Product Specific Information General Information
PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms.

PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein.

PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal.

Figure 1 shows a Western blot of pdx-1 in human pancreatic cancer cells MIA-1, PANC-1 and CAPAN-1 using PA3-830.

Figure 2 shows immunofluorescence staining of pdx-1 in mouse islet cells using PA3-830 and counterstaining (merge) with insulin. The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended.
pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription.

Additionally, pdx-1 has been studied for its role in pancreatic development which includes maturation and differentiation of pancreatic precursor cells. Mutations in the pdx-1 gene have been shown to cause pancreatic agenesis, maturity-onset diabetes of the young, and type II diabetes.
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References:
World J Surg. 2005 Mar;29(3):334-8.
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PDX-1 Polyclonal Antibody (PA3-831)
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