Anti-PDX-1 Polyclonal Antibody
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Synonyms:
Pyruvate Dehydrogenase Complex Component-X, PDHX, E3BP, DLDBP, Pyruvate Dehydrogenase Complex Lipoyl-Containing Component X
Entrez Gene ID:
(Human) 3651, (Murine) 18609
UniProt ID:
P52945
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Murine (Ms) |
| Immunogen: | Synthetic Peptide: Q(21) R G P A P E F S A S P P A C(35) |
| Ordering Information | ||||
| Pierce Anti-PDX-1 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA3-830 | 100 µl | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ul of antiserum containing 0.05% sodium azide. |
| Applications | Dilution |
| Immunofluorescence (IF) | 1:500 |
| Immunohistochemistry (IHC) | 1:500 |
| Western Blot (WB) | 1:3,000 |
| Product Specific Information |
| PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms. PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein. PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal. Figure 1 shows a Western blot of pdx-1 in human pancreatic cancer cells MIA-1, PANC-1 and CAPAN-1 using PA3-830. Figure 2 shows immunofluorescence staining of pdx-1 in mouse islet cells using PA3-830 and counterstaining (merge) with insulin. The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended. |
| General Information |
| pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription. Additionally, pdx-1 has been studied for its role in pancreatic development which includes maturation and differentiation of pancreatic precursor cells. Mutations in the pdx-1 gene have been shown to cause pancreatic agenesis, maturity-onset diabetes of the young, and type II diabetes. |
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Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830)
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| References: |
| World J Surg. 2005 Mar;29(3):334-8. |
(This product is for In Vitro experimental use only.)
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