Anti-PDX-1 Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) Murine (Ms) |
| Immunogen: |
Synthetic Peptide: Q(21) R G P A P E F S A S P P A C(35) |
|
| Immunofluorescence (IF) |
1:500 |
| Immunohistochemistry (IHC) |
1:500 |
| Western Blot (WB) |
1:3,000 |
| Pierce Anti-PDX-1 Polyclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
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| PA3-830 |
100 µl |
$354.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 ul of antiserum containing 0.05% sodium azide. |
PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms.
PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein.
PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal.
Figure 1 shows a Western blot of pdx-1 in human pancreatic cancer cells MIA-1, PANC-1 and CAPAN-1 using PA3-830.
Figure 2 shows immunofluorescence staining of pdx-1 in mouse islet cells using PA3-830 and counterstaining (merge) with insulin. The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended. |
pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription.
Additionally, pdx-1 has been studied for its role in pancreatic development which includes maturation and differentiation of pancreatic precursor cells. Mutations in the pdx-1 gene have been shown to cause pancreatic agenesis, maturity-onset diabetes of the young, and type II diabetes. |
 
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This product is for In Vitro experimental use only
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