Anti-Cyclophilin D Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
|
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: |
Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40. |
|
| Flow Cytometry (FACS) |
Assay dependent |
| Gel Shift (GS) |
Assay dependent |
| Immunocytochemistry (ICC) |
Assay dependent |
| Immunohistochemistry (Frozen) (IHC (F)) |
Assay dependent |
| Immunoprecipitation (IP) |
Assay dependent |
| Western Blot (WB) |
1:1,000 |
| Pierce Anti-Cyclophilin D Polyclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
|
| PA3-022 |
100 µl |
$354.00 |
|
|
|
|
| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µl of antiserum diluted in PBS containing 0.05% sodium azide. |
PA3-022 detect cyclophilin 40 (CyP-40) from human and rat and mouse tissues and cells. This antibody does not cross-react with CyPA.
PA3-022 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, gel shift and FACS procedures. By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.
The PA3-022 immunogen is a synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40.
Figure 1 shows a Western blot of Cyp 40 on rat spleen extracts using PA3-022. |
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues. |
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| Biochem. J., 307: 5-8, 1995. |
| Mol. Ednocrinol., 9(7): 838-847, 1995. |
| J. Biol. Chem., 270(35): 20479-20484, 1995. |
| Cell, 94: 471-480, 1998. |
| J. Biol. Chem., 267(8): 5503-5507, 1992. |
| J. Biol. Chem., 270(32): 18841-18847, 1995. |
| J. Biol. Chem., 268(17): 12303-12310, 1993. |
| Mol. Endocrinol. Apr 2005 doi:10.1210/me.2005-0071 |
| Mol. Cell. Biol., Mar 1998; 18: 1517 - 1524. |
This product is for In Vitro experimental use only
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