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PROTEASOME 19S SUBUNIT S7 ANTIBODIES > PROTEASOME 19S SUBUNIT S7 POLYCLONAL ANTIBODY PA1-969
Anti-Proteasome 19S Subunit S7 Polyclonal Antibody
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Synonyms:
MGC3004, MSS1, Nbla10058, S7
Entrez Gene ID:
(Human) 5701
UniProt ID:
P35998
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Human recombinant proteasome 19S subunit S7. |
| Ordering Information | ||||
| Pierce Anti-Proteasome 19S Subunit S7 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-969 | 100 µl | $350.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of purified total IgG in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1:1,000 |
| Product Specific Information |
| PA1-969 detects proteasome 19S subunit S7 from human cells. PA1-969 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate. PA1-969 antigen is recombinant human proteasome 19S subunit S7. Figure 1 shows a Western blot of proteasome 19S subunit S7 on HeLa cell lysate using PA1-969. |
| General Information |
| Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome. |
| References: |
| Biochem. 68:1015-1068, 1999. |
| Annu. Rev. Med. 50:57-74, 1999. |
| Annu. Rev. Cell Biol., 3:1-30, 1987. |
(This product is for In Vitro experimental use only.)
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