Part of Thermo Fisher Scientific

Anti-S100 alpha Polyclonal Antibody

Printable Datasheet

Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Clone:
Label:
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Bovine (Bv) Porcine (Po)
Immunogen: Purified S100-alpha protein from human pectoral muscle cells.
Applications Dilution
ELISA (ELISA) Assay dependent
Immunohistochemistry (Paraffin) (IHC (P)) 1 ug/ml
Immunoprecipitation (IP) 5 ug/ml
Western Blot (WB) 0.1 ug/ml
Ordering Information
Pierce Anti-S100 alpha Polyclonal Antibody
Product Number Pkg Size Price


PA1-932 100 µg $350.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µg of epitope affinity purified IgG lyophilized from PBS containing 15 mg/ml BSA and 0.05% sodium azide.


Product Specific Information General Information
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.

PA1-932 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.

S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.

The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.

Figure 1 shows a Western blot of S100-alpha from human muscle extract using PA1-932.
The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells.
Image
  
References:
Neurochem. Int., 6(4): 513-518, 1984
Biomed. Res., 8(2): 119-125, 1987
Similar Products:
Calbindin D-28K Polyclonal Antibody (PA1-931)
Parvalbumin Polyclonal Antibody (PA1-933)
S100 alpha Monoclonal Antibody (MA1-90602)
This product is for In Vitro experimental use only
Learn more about other Thermo Scientific Life Science Research Products
Protein Research Products Cell Culture High Content Screening  
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies  European Sales

 © 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723  or  815-968-0747

Trademarks Privacy Statement