Anti-S100 alpha Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) Bovine (Bv) Porcine (Po) |
| Immunogen: |
Purified S100-alpha protein from human pectoral muscle cells. |
|
| ELISA (ELISA) |
Assay dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) |
1 ug/ml |
| Immunoprecipitation (IP) |
5 ug/ml |
| Western Blot (WB) |
0.1 ug/ml |
| Pierce Anti-S100 alpha Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
|
| PA1-932 |
100 µg |
$350.00 |
|
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µg of epitope affinity purified IgG lyophilized from PBS containing 15 mg/ml BSA and 0.05% sodium azide. |
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.
PA1-932 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.
S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.
Figure 1 shows a Western blot of S100-alpha from human muscle extract using PA1-932. |
The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells. |
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This product is for In Vitro experimental use only
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