Part of Thermo Fisher Scientific

Anti-S100 alpha Polyclonal Antibody

Synonyms:
S100alpha
Entrez Gene ID:
(Human) 6271, (Murine) 20193, (Rat) 295214
UniProt ID:
P23297
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Bovine (Bv) Porcine (Po)
Immunogen: Purified S100-alpha protein from human pectoral muscle cells.
Ordering Information
Pierce Anti-S100 alpha Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA1-932 100 µg $350.00


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µg of epitope affinity purified IgG lyophilized from PBS containing 15 mg/ml BSA and 0.05% sodium azide.


Applications Dilution
ELISA (ELISA) Assay dependent
Immunohistochemistry (Paraffin) (IHC (P)) 1 ug/ml
Immunoprecipitation (IP) 5 ug/ml
Western Blot (WB) 0.1 ug/ml
Product Specific Information
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.

PA1-932 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.

S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.

The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.

Figure 1 shows a Western blot of S100-alpha from human muscle extract using PA1-932.
General Information
The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells.
References:
Neurochem. Int., 6(4): 513-518, 1984
Biomed. Res., 8(2): 119-125, 1987
(This product is for In Vitro experimental use only.)


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