| Details | |
| Host / Isotype: | Goat |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide corresponding to the C terminus amino acids KSFKANLENPIRLA. |
| Ordering Information | ||||
| Pierce Anti-PDHX Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-9090 | 100 ug | $322.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of affinity purified antibody in 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Applications | Dilution |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1-3 µg/ml |
| Western Blot (WB ) | 0.1-1 µg/ml |
| Product Specific Information |
| PA1-9090 detects PDHX in human samples. PA1-9090 has been successfully used in Western blot and immunohistochemistry (paraffin-embedded sections) procedures. PA1-9090 immunogen is a synthetic peptide corresponding to the C terminus of PDHX, amino acids KSFKANLENPIRLA. Figure 1 illustrates specific staining of paraffin embedded human pancreas using PA1-9090. A) Microwaved antigen retrieval with citrate buffer pH 6 (3µg/ml), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1µg/ml) , HRP-staining |
| General Information |
| Pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription. |
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