| Details | |
| Host / Isotype: | Goat |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide corresponding to the C terminus amino acids CKEKATRPSQGHQP. |
| Ordering Information | ||||
| Pierce Anti-Choline Acetyltransferase Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-9027 | 100 ug | $268.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of affinity purified antibody (0.5 mg/ml) in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Applications | Dilution |
| ELISA (ELISA) | 1:32000 |
| Western Blot (WB ) | 0.03-0.3 µg/ml |
| Product Specific Information |
| PA1-9027 detects CHAT in human samples. Human, canine, and porcine samples are expected to cross react due to sequence similarity. PA1-9027 has been successfully used in Western blot procedures. In Western blot an ~70kDa band is observed in human placenta lysates. PA1-9027 immunogen is a synthetic peptide corresponding to the C terminus of CHAT, amino acids CKEKATRPSQGHQP. Figure 1 illustrates Western blot detection of ChAT in human Placenta lysate using PA1-9027. |
| General Information |
| This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. |
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Box 117, Rockford, IL 61105 USA |
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