Anti-HAI-2 Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
|
| Species Reactivity: |
Human (Hu) |
| Immunogen: |
Synthetic peptide based on the cytoplasmic domain of human HAI-2. |
|
| Western Blot (WB) |
1:5,000 |
| Pierce Anti-HAI-2 Polyclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
|
| PA1-8464 |
100 µg |
$295.00 |
|
|
|
|
| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 0.05% sodium azide and 50% glycerol. |
PA1-8464 detects HAI-2 from human samples. This antibody does not cross react with HAI-1.
PA1-8463 has been successfully used in Western blotting procedures. By Western blot, this antibody detects ~32 kDa proteins representing HAI-2.
PA1-8464 immunizing peptide is based on the cytoplasmic domain of human HAI-2. |
HAI-2 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HAI-2 also inhibits trypsin, and MTSP-1 (Matriptase-1), a serine proteinase that also activates HGF, and HGF-2 has been shown to inhibit hepsin with nanamolar kIs. HAI-2 was first known as bikunin, and SPINT2 (Serine Proteinase Inhibitor-2), a proteinase inhibitor found in amniotic fluid. A protein termed HAI-2 related small protein is a 106 amino acid protein with little homology to HAI-2, and the HAI-2 antibodies do not recognize the HAI-2 RSP.
The chondroitin sulfate groups attached to HAI-2 are thought to be important for its function. The HAI-2 antibodies do not recognize HAI-1. Mice treated with HAI-2 in an ovarian cancer model had smaller tumors, and HAI-2 is reported to be elevated in macrophages during ovarian cancer, and this is considered a favorable indicator of survival. HAI-2 is secreted and membrane-inserted via a type-I transmembrane domain. The carboxyterminal end is a cytoplasmic domain, thought to interact with signaling and cytoskeletal machinery. The extracellular domains include two kringle domains (KD1 and KD2), and KD2 is thought to be inactive or with much lower activity against HGF. In mice the dominant HAI-2 lacks the first kringle domain, but the two-domain form dominates in humans, although in human testis a shorter HAI-2 has been reported, using a second transcription site. |
No references for this product. |
This product is for In Vitro experimental use only
Printable Datasheet
