Anti-GRIP1 Polyclonal Antibody
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Synonyms:
Glucocorticoid Receptor Interacting Protein, Transcription Intermediary Factor 2, TIF 2
Entrez Gene ID:
(Human) 23426
UniProt ID:
Q9Y3R0
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Hamster (Hm) |
| Immunogen: | Synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1. |
| Ordering Information | ||||
| Pierce Anti-GRIP1 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-846 | 100 µg | $350.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Immunocytochemistry (ICC) | 1:1000 |
| Immunofluorescence (IF) | 1:1000 |
| Product Specific Information |
| PA1-846 detects glucocorticoid receptor interacting protein-1 (GRIP1) from hamster and human cells. PA1-846 has been successfully used in immunocytochemistry, and immunofluorescence procedures. Immunocytochemical staining of GRIP1 in CHO cells with PA1-846 results in cytoplasmic and nuclear staining. This antibody has not been used successfully in Western blot procedures. PA1-846 immunogen is a synthetic peptide corresponding to residues P(206) L P D S E E E G H D S(217) C of human GRIP1. The PA1-846 immunizing peptide (Cat. # PEP-050) is available for use in neutralization and control experiments. Figure 1 shows immunolocalization of GRIP1 in CHO cells using PA1-846. |
| General Information |
| Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Glucocorticoid receptor interacting protein-1 (GRIP1), or Transcription Intermediary Factor 2 (TIF2), is a member of a family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC-1) and Amplified in breast cancer-1 (AIB-1). GRIP1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, estrogen, progesterone, and peroxisome proliferator activated receptors. GRIP1 also contains several copies of the conserved LXXLL/LLXXL motif which has been demonstrated to be critical to receptor-coactivator interactions. |
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Immunofluorescence with anti-GRIP1 Polyclonal Antibody (PA1-846)
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| References: |
| J. Bio. Chem., 274(3): 3496-3502, 1999. |
| JBC 277(40):37037-37044, 2002. |
| Nucleic Acids Research, 26(5): 1191-1197, 1998. |
| Molec.& Cell. Biol., 17(3): 2735-2744, 1997. |
(This product is for In Vitro experimental use only.)
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