Anti-c-Jun Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Murine (Ms) |
| Immunogen: |
Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun. |
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| Western Blot (WB) |
10 µg/ml |
| Pierce Anti-c-Jun Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
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| PA1-833 |
200 µg |
$350.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
200 µg of lyophilized, purified rabbit IgG, containing 0.05% sodium azide. Reconstitute with PBS. |
PA1-833 detects cJun from mouse cells.
PA1-833 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun.
The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.
Figure 1 shows cJun in NIH 3T3 cells using PA1-833 after serum stimulation. |
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes. |
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This product is for In Vitro experimental use only
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