Part of Thermo Fisher Scientific

Anti-c-Jun Polyclonal Antibody

Synonyms:
Jun, Jun Oncogene
Entrez Gene ID:
(Murine) 16476
UniProt ID:
P05627
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Murine (Ms)
Immunogen: Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.
Ordering Information
Pierce Anti-c-Jun Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA1-833 200 µg $350.00


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 200 µg of lyophilized, purified rabbit IgG, containing 0.05% sodium azide. Reconstitute with PBS.


Applications Dilution
Western Blot (WB) 10 µg/ml
Product Specific Information
PA1-833 detects cJun from mouse cells.

PA1-833 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun.

The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.

Figure 1 shows cJun in NIH 3T3 cells using PA1-833 after serum stimulation.
General Information
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.
References:
Critical Reviews in Eukaryotic Gene Expression, 4(1): 55-116, 1994.
Biochimica et Biophysica Acta, 1072: 129-157, 1991.
(This product is for In Vitro experimental use only.)


Learn more about other Thermo Scientific Life Science Research Products
Protein Research Products Cell Culture High Content Screening
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies European Sales

© 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723 or 815-968-0747

Trademarks Privacy Statement