Anti-c-Jun Polyclonal Antibody
Printable Datasheet
Synonyms:
Jun, Jun Oncogene
Entrez Gene ID:
(Murine) 16476
UniProt ID:
P05627
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun. |
| Ordering Information | ||||
| Pierce Anti-c-Jun Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-833 | 200 µg | $350.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 200 µg of lyophilized, purified rabbit IgG, containing 0.05% sodium azide. Reconstitute with PBS. |
| Applications | Dilution |
| Western Blot (WB) | 10 µg/ml |
| Product Specific Information |
| PA1-833 detects cJun from mouse cells. PA1-833 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun. The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun. Figure 1 shows cJun in NIH 3T3 cells using PA1-833 after serum stimulation. |
| General Information |
| Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes. |
| References: |
| Critical Reviews in Eukaryotic Gene Expression, 4(1): 55-116, 1994. |
| Biochimica et Biophysica Acta, 1072: 129-157, 1991. |
(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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