Anti-VAMP2 Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Murine (Ms) Rat (Rt) |
| Immunogen: |
Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2. |
|
| Immunoprecipitation (IP) |
Assay dependent |
| Western Blot (WB) |
0.3 µg/ml |
| Pierce Anti-VAMP2 Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
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| PA1-766 |
100 µg |
$358.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
PA1-766 detects vesicle associated membrane protein 2 (VAMP-2) from rat and mouse tissues.
PA1-766 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 19 kDa protein representing VAMP-2 from rat brain whole protein extract.
The PA1-766 immunogen is a synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-101) is available for use in neutralization and control experiments.
Figure 1 shows a Western blot of VAMP-2 on rat brain extract using PA1-766. |
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin. |
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This product is for In Vitro experimental use only
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