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Anti-VAMP2 Polyclonal Antibody

Printable Datasheet

Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Clone:
Label:
Species Reactivity: Murine (Ms) Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2.
Applications Dilution
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 0.3 µg/ml
Ordering Information
Pierce Anti-VAMP2 Polyclonal Antibody
Product Number Pkg Size Price


PA1-766 100 µg $358.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.


Product Specific Information General Information
PA1-766 detects vesicle associated membrane protein 2 (VAMP-2) from rat and mouse tissues.

PA1-766 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 19 kDa protein representing VAMP-2 from rat brain whole protein extract.

The PA1-766 immunogen is a synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-101) is available for use in neutralization and control experiments.

Figure 1 shows a Western blot of VAMP-2 on rat brain extract using PA1-766.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
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References:
Cell Tissue Res. 296:499-510, 1999.
AJP Cell Physiol doi:10.1152/ajpcell.00241.2004 Sept 2004
Mol. Endo. 19(1):213-224, 2005.
Mol Cell Biology Vol 22, No 1, 378-387, Jan 2002
Mol. Cell. Biol., Vol 22: 378-387, Jan 2002
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This product is for In Vitro experimental use only
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