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PDE6 BETA ANTIBODIES > PDE6 BETA POLYCLONAL ANTIBODY PA1-722
Anti-PDE6 beta Polyclonal Antibody
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Synonyms:
Phosphodiesterase 6 Beta
Entrez Gene ID:
(Murine) 18587
UniProt ID:
P23440
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic Peptides: H(20) Q Y F G (K/R) K L S P E N V A G A C(36) |
| Ordering Information | ||||
| Pierce Anti-PDE6 beta Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-722 | 100 µg | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of epitope affinity purified IgG in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Immunohistochemistry (IHC) | Assay dependent |
| Western Blot (WB) | 2 ug/ml |
| Product Specific Information |
| PA1-722 detects phosphodiesterase 6 beta (PDE6 beta) from mouse retinal extracts. PA1-722 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 beta from mouse retinal extracts. The PA1-722 immunizing peptides correspond to amino acid residues 20-36 from mouse and bovine PDE6 beta protein. These peptides (Cat. # PEP-183) are available for use in neutralization and control experiments. Figure 1 shows a Western blot of PDE6 beta using PA1-722. Lane 1 demostrates no detection on extracts of RD mice. Lane 2 shows a single band at ~90 kDa on Balb/C extracts. Figure 2 shows immunoflourences of mouse retina outer segments after staining with PA1-722. |
| General Information |
| The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha’ (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha’/alpha’ homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association. |
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Immunofluorescence with anti-PDE6 beta Polyclonal Antibody (PA1-722)
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(This product is for In Vitro experimental use only.)
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