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PDE6 ALPHA' ANTIBODIES > PDE6 ALPHA' POLYCLONAL ANTIBODY PA1-721
Anti-PDE6 alpha' Polyclonal Antibody
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Synonyms:
Phosphodiesterase 6 Alpha'
Entrez Gene ID:
(Bovine) 5146
UniProt ID:
P51160
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Bovine (Bv) Ovine (Ov) |
| Immunogen: | Synthetic peptide corresponding to residues M(16) P Q F A K E Y F D R K L R(29) C of human PDE6 alpha’. |
| Ordering Information | ||||
| Pierce Anti-PDE6 alpha' Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-721 | 100 µl | $350.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of purified IgG in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | 1:500 |
| Product Specific Information |
| PA1-721 detects phosphodiesterase 6 alpha’ (PDE6 alpha’) from sheep and bovine retinal extract. PA1-721 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 alpha’ from sheep and bovine retinal extract. The PA1-721 immunogen is a synthetic peptide corresponding to residues M(16) P Q F A K E Y F D R K L R(29) C of human PDE6 alpha’. This sequence is completely conserved between human, chicken and frog. PA1-721 immunizing peptide (Cat. # PEP-149) is available for use in neutralization and control experiments. Figure 1 shows a Western blot of PDE6 alpha’ on sheep retinal extracts using PA1-721. |
| General Information |
| The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha’ (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha’/alpha’ homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association. |
(This product is for In Vitro experimental use only.)
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