Anti-LAMP2 Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
|
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: |
Synthetic Peptide: C G(400) L K R H H T G Y E Q F(411) |
|
| Immunofluorescence (IF) |
Assay dependent |
| Western Blot (WB) |
1:500 |
| Pierce Anti-LAMP2 Polyclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
|
| PA1-655 |
100 µl |
$354.00 |
|
|
|
|
| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µl of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
PA1-655 detects lysosome-associated membrane protein-2 (LAMP2) from rat, mouse and human tissues.
PA1-655 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing LAMP2 from purified lysosomes from L cells.
PA1-655 immunizing peptide corresponds to amino acid residues 400-411 from rat LAMP2. This sequence is completely conserved between rat, mouse, and guinea pig LAMP2. PA1-655 immunizing peptide (Cat. # PEP-039) is available for use in neutralization and control experiments. |
Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome-associated membrane protein-2 (LAMP-2) is an ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. |
| J. Cell Biol., 101: 85-95, 1985. |
| J. Cell Biol., 137: 1161-1169, 1997. |
| Molecular and Cellular Biology Vol. 25, no. 3 1025-1040, 2005 |
| MBC Vol. 16, 1696-1710 2005 |
| JBC Vol 279, No 49, 51315-51322, Dec 2004 |
| Carcinogenesis, Mar 2007; 28: 537 - 544. |
This product is for In Vitro experimental use only
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