Anti-ECT Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) Non-human primate (Nhp) |
| Immunogen: |
Synthetic Peptide: C(157) T K A H H S S Q E M S S E Y R E(172) |
|
| Western Blot (WB) |
1 ug/ml |
| Pierce Anti-ECT Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
|
| PA1-545 |
100 µg |
$350.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 ug of affinity purified antibody (0.4 mg/ml for a total volume of 250 ul) in Tris glycine buffer containing 1 mg/ml BSA and 0.02% thimerosal. |
PA1-545 detects ethanolaminephosphate cytidyltransferase (ECT) protein in human, rat, mouse and non-human primate samples.
PA1-545 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing ECT from C3H/10T1/2 cell lysates.
PA1-545 immunizing peptide corresponds to amino acid residues 157-172 from human ECT protein. This immunizing peptide (PEP-241) can be used for neutralization and control experiments.
Figure 1 shows a Western blot of ECT in COS-7 cells using PA1-545.
Figure 2 shows a Western blot of ECT in C3H/10T1/2 cells using PA1-545. |
ECT (ethanolaminephosphate cytidyltransferase) is an important regulatory enzyme in the pathway for phosphatidylethanolamine (PE) biosynthesis. PE functions in cell signalling, membrane fusion, platelet activation, and cell cycle progression. Specialized forms of PE exist as plasmologens, natural cannabinoid anadimade, and glycosylated PE. The ECT enzyme acts in the second of three catalysis steps to produce CDP-ethanolamine from phosphoethanolamine (P-Etn). Study of ECT could help determine the role of plasmologens in disease, specifically lipid disorders such as peroxisomal diseases as well as neuronal degeneration associated with Alzheimer's disease. |
 
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No references for this product. |
This product is for In Vitro experimental use only
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