Part of Thermo Fisher Scientific

Anti-ECT Polyclonal Antibody

Synonyms:
Ethanolaminephosphate Cytidyltransferase,
Entrez Gene ID:
(Human) 5833
UniProt ID:
Q99447
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Non-human primate (Nhp)
Immunogen: Synthetic Peptide: C(157) T K A H H S S Q E M S S E Y R E(172)
Ordering Information
Pierce Anti-ECT Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA1-545 100 µg $350.00


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ug of affinity purified antibody (0.4 mg/ml for a total volume of 250 ul) in Tris glycine buffer containing 1 mg/ml BSA and 0.02% thimerosal.


Applications Dilution
Western Blot (WB) 1 ug/ml
Product Specific Information
PA1-545 detects ethanolaminephosphate cytidyltransferase (ECT) protein in human, rat, mouse and non-human primate samples.

PA1-545 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing ECT from C3H/10T1/2 cell lysates.

PA1-545 immunizing peptide corresponds to amino acid residues 157-172 from human ECT protein. This immunizing peptide (PEP-241) can be used for neutralization and control experiments.

Figure 1 shows a Western blot of ECT in COS-7 cells using PA1-545.

Figure 2 shows a Western blot of ECT in C3H/10T1/2 cells using PA1-545.
General Information
ECT (ethanolaminephosphate cytidyltransferase) is an important regulatory enzyme in the pathway for phosphatidylethanolamine (PE) biosynthesis.  PE functions in cell signalling, membrane fusion, platelet activation, and cell cycle progression.  Specialized forms of PE exist as plasmologens, natural cannabinoid anadimade, and glycosylated PE.  The ECT enzyme acts in the second of three catalysis steps to produce CDP-ethanolamine from phosphoethanolamine (P-Etn).  Study of ECT could help determine the role of plasmologens in disease, specifically lipid disorders such as peroxisomal diseases as well as neuronal degeneration associated with Alzheimer's disease.
(This product is for In Vitro experimental use only.)


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