Part of Thermo Fisher Scientific

Anti-TAF4p Polyclonal Antibody

Synonyms:
Taf48p, Tsg2p, Mpt1p
Entrez Gene ID:
(Yeast) 855017
UniProt ID:
P50105
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Yeast (Ys)
Immunogen: the N-terminal domain of Taf4p protein (amino acid residues 1-200)
Ordering Information
Pierce Anti-TAF4p Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA1-4937 250 µl $845.00


Storage: 4º C
Form: 250 µl of chromatography purified antibody with 0.1% sodium azide.


Applications Dilution
ELISA (ELISA) Assay dependent
Western Blot (WB) Assay dependent
Product Specific Information
PA1-4937 detects Taf4p in budding yeast samples.

PA1-4937 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~48 kDa protein (Taf4p).

PA1-4937 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the N-terminal domain of Taf4p protein (amino acid residues 1-200).

Figure 1 illustrates detection of Taf4p via Western blotting using the Taf4p antibody. Lane 2: Extract of budding yeast.
General Information
The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time, it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf4p is one of the subunits of TFIID and in the case of budding yeast, it is composed of 388 amino acid residues (aa). This protein contains histone folds in its interior and forms TAF octamer with Taf6p, Taf9p and Taf12p.
(This product is for In Vitro experimental use only.)


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