Anti-RAD51 Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
|
| Species Reactivity: |
Bacteria (Ba) |
| Immunogen: |
Recombinant RecA expressed in E. coli. |
|
| ELISA (ELISA) |
Assay dependent |
| Immunoprecipitation (IP) |
Assay dependent |
| Western Blot (WB) |
1:1000 |
| Pierce Anti-RAD51 Polyclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
|
| PA1-4925 |
250 µg |
$845.00 |
|
|
|
|
| Storage: |
-80º C, Avoid Freeze/Thaw Cycles |
| Form: |
250 µg of chromatography purified antibody with 0.05% sodium azide. |
PA1-4925 detects RecA in E. coli samples.
PA1-4925 has been successfully used in Western blot, ELISA, and immunoprecipitation procedures. By western blot the antibody detects a ~38 kDa protein (RecA) in E. coli samples.
PA1-4925 immunogen corresponds to recombinant RecA protein
Recombinant RecA protein (Cat. # RP-4900 or RP-4901) can be purchased for control experiments in Western blot assays.
Figure 1 illustrates detection of Gal11p by Western blotting using the Gal11p antibody. Lane 2: Extract of budding yeast. |
E. coli RecA protein plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its co-protease activity after DNA damage, and induces many proteins related to DNA repair, including RecA itself .
Since RecA has many functions and interacts with various proteins in the cell, the protein complexes can be detected by immunoprecipitaion with the anti-RecA antibody.
RecA forms foci on nucleoids in the cells after exposure to DNA damaging treatments. The foci is visualized by immuno-staining of the cells using this antibody as the first antibody. |
|
No references for this product. |
This product is for In Vitro experimental use only
Printable Datasheet
