Anti-RAD51 Polyclonal Antibody
Printable Datasheet
Synonyms:
RAD51A
Entrez Gene ID:
(Bacteria) 5888
UniProt ID:
Q06609
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Bacteria (Ba) |
| Immunogen: | Recombinant RecA expressed in E. coli. |
| Ordering Information | ||||
| Pierce Anti-RAD51 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-4925 | 250 µL | $845.00 | ||
| Storage: | 4º C |
| Form: | 250 µL of antiserum with 0.05% sodium azide. |
| Applications | Dilution |
| ELISA (ELISA) | Assay dependent |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1:1000 |
| Product Specific Information |
| PA1-4925 detects RecA in E. coli samples. PA1-4925 has been successfully used in Western blot, ELISA, and immunoprecipitation procedures. By western blot the antibody detects a ~38 kDa protein (RecA) in E. coli samples. PA1-4925 immunogen corresponds to recombinant RecA protein. Store at 4ºC short term or at -80ºC for long term. Figure 1 illustrates detection of RAD51 by Western blot. |
| General Information |
| E. coli RecA protein plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its co-protease activity after DNA damage, and induces many proteins related to DNA repair, including RecA itself . Since RecA has many functions and interacts with various proteins in the cell, the protein complexes can be detected by immunoprecipitaion with the anti-RecA antibody. RecA forms foci on nucleoids in the cells after exposure to DNA damaging treatments. The foci is visualized by immuno-staining of the cells using this antibody as the first antibody. |
(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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