| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Rat (Rt) |
| Immunogen: | Synthetic peptide corresponding to the C-terminal region of rat NSF protein. |
| Ordering Information | ||||
| Pierce Anti-NSF Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-4690 | 100 µl | $285.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of affinity purified antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. |
| Applications | Dilution |
| Western Blot (WB) | 1/1000 |
| Product Specific Information |
| PA1-4690 detects NSF protein in rat samples. It is anticipated that the antibody will also work with human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen. PA1-4690 has been successfully used in Western blotting. By Western blot, this antibody is specific for ~75 kDa NSF protein. PA1-4690 is a peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH). Figure 1 shows a Western blot of rat caudate lysate showing specific labeling of the ~75 kDa NSF protein. |
| General Information |
| NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004). |
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