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PHOSPHO-RAF1 (SER301) ANTIBODIES > PHOSPHO-RAF1 (SER301) POLYCLONAL ANTIBODY PA1-4639
Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody
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Synonyms:
Craf 1 transforming gene, Craf1 transforming gene, Murine sarcoma 3611 oncogene 1, Raf proto oncogene serine/threonine protein kinase, vraf1 murine le
Entrez Gene ID:
(Human) 5894, (Murine) 110157, (Rat) 24703
UniProt ID:
P04049
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Murine (Ms) Rat (Rt) Xenopus laevis (Xl) |
| Immunogen: | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf-1. |
| Ordering Information | ||||
| Pierce Anti-Phospho-RAF1 (Ser301) Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-4639 | 100 µl | $285.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ul of affinity purified IgG in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. |
| Applications | Dilution |
| Western Blot (WB) | 1:1000 |
| Product Specific Information |
| PA1-4639 detects Phospho-Raf-1 (Ser301) in rat, mouse, human, and Xenopus samples. PA1-4639 has successfully been used in Western blotting. It is specific for the ~74 kDa Raf-1 protein phosphorylated at Ser301. Immunolabeling of the Raf-1 protein band is blocked by lambda-phosphatase treatment. PA1-4639 immunogen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf-1. The sequence of the immunogen is identical in rat, mouse, human, and Xenopus. Figure 1 shows a Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74 kDa Raf-1 protein (Lane 1). The phosphospecificty of this labeling is shown in Lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser301 Raf-1. The immunolabeling of Raf-1 is completely eliminated by treatment with lambda-Ptase. |
| General Information |
| The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chon et al, 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al, 2005) with two sites participating Ser 301 and Ser 642. |
(This product is for In Vitro experimental use only.)
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