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Anti-Phospho-PAK1,2,3 (Thr402) Polyclonal Antibody

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Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Clone:
Label:
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Non-human primate (Nhp)
Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 Activated Kinase 2 (PAK-2).
Applications Dilution
Western Blot (WB) 1:1000
Ordering Information
Pierce Anti-Phospho-PAK1,2,3 (Thr402) Polyclonal Antibody
Product Number Pkg Size Price


PA1-4636 100 µl $342.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ul of affinity purified IgG in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.


Product Specific Information General Information
PA1-4636 detects Phospho-PAK-1,2,3 (Thr402) in rat, mouse, human, and non-human primate samples.

PA1-4636 has successfully been used in Western blotting. It is specific for the ~68 kDa to ~70 kDa PAK phosphorylated at Thr402. The immunolabeling of the PAK protein band is blocked by lambda-phosphatase treatment.

PA1-4636 immunogen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 Activated Kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. The sequence of the immunogen is identical in rat, mouse, human, and non-human primate.

Figure 1 shows a Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein (Lane 1). The phosphospecificty of this labeling is shown in Lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Phospho- Thr402 PAK-1,2,3. The immunolabeling of PAK is completely eliminated by treatment with lambda-phosphatase.
In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al.,
1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
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