Anti-CDKN2A Polyclonal Antibody
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Synonyms:
RP23-166I8.5, ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19, p19ARF
Entrez Gene ID:
(Murine) 12578
UniProt ID:
P51480
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF. [Swiss-Prot #P51480] |
| Ordering Information | ||||
| Pierce Anti-CDKN2A Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-46220 | 100 µl | $342.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of affinity purified antisera (1 mg/ml) in Tris-citrate/phosphate [pH 7-8] containing 0.1% sodium azide. |
| Applications | Dilution |
| ELISA (ELISA) | Assay Dependent |
| Immunocytochemistry (ICC) | 1 µg/ml |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/ml |
| Western Blot (WB) | 2 µg/ml |
| Product Specific Information |
| The PA1-46220 antibody reacts with p19ARF in murine samples. The immunogen used for this production shares 80% homology with human protein, 93% with rat protein, and 86% with porcine protein. PA1-46220 has been successfully used in ELISA, immunohistochemical, immunocytochemical and Western Blot applications. By Western blot, this antibody detects a ~19 kDa band. The PA1-46220 antibody was raised against a synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF. [Swiss-Prot #P51480]. |
| General Information |
| The INK4a/ARF locus encodes two unrelated tumor suppressor proteins, p16INK4a and p19ARF. These proteins restrain cell growth by modifying the functions of the retinoblastoma protein and p53, respectively. INK4a/ARF is among the most frequently mutated tumor suppressor loci in human cancer. Both p16INK4a and p19ARF act as cell proliferation inhibitors. The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction. |
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Western Blot with anti-CDKN2A Polyclonal Antibody (PA1-46220)
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Western Blot with anti-CDKN2A Polyclonal Antibody (PA1-46220)
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(This product is for In Vitro experimental use only.)
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PO
Box 117, Rockford, IL 61105 USA |
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