Anti-CDKN2A Polyclonal Antibody
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Synonyms:
CDKN2A
Entrez Gene ID:
(Human) 1029, (Murine) 12578, (Rat) 25163
UniProt ID:
P42771
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide corresponding to the C-terminus of human p14ARF. |
| Ordering Information | ||||
| Pierce Anti-CDKN2A Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-38334 | 1 ml | $388.00 | ||
| Storage: | 4º C |
| Form: | 1 ml of epitope affinity purified antibody (0.2 mg/ml) in 10mM PBS, pH 7.4 containing sodium azide and BSA. |
| Applications | Dilution |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 for 30 min @ RT |
| Western Blot (WB) | 1-2µg/ml for 2 hrs |
| Product Specific Information |
| PA1-38334 detects p14 ARF from human samples. PA1-38334 has been successfully used in immunohistochemistry (paraffin) and Western blot applications. The PA1-38334 immunogen is a synthetic peptide corresponding to the C-terminus of human p14ARF. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minute followed by cooling at room temperature for 20 minutes. |
| General Information |
| The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 proteinrequired for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1Beta-encoded N-terminal domain of p14ARF and a C-terminal region of mdm2. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways. |
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Immunohistochemistry with anti-CDKN2A Polyclonal Antibody (PA1-38334)
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(This product is for In Vitro experimental use only.)
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PO
Box 117, Rockford, IL 61105 USA |
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