Part of Thermo Fisher Scientific
HOME >
CYTOCHROME P450 3A4 ANTIBODIES > CYTOCHROME P450 3A4 POLYCLONAL ANTIBODY PA1-343
Anti-Cytochrome P450 3A4 Polyclonal Antibody
Printable Datasheet
Synonyms:
CYP, P450, Quinine 3-monooxygenase, CYPIIIA4, Nifedipine oxidase, Taurochenodeoxycholate 6-alpha-hydroxylase, NF-25, P450-PCN1
Entrez Gene ID:
(Human) 1576
UniProt ID:
P08684
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Rat (Rt) |
| Immunogen: | Purified, His-tagged, full length human P450 3A4 fusion protein. |
| Ordering Information | ||||
| Pierce Anti-Cytochrome P450 3A4 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-343 | 100 µl | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of purified IgG in PBS containing 1.0 mg/ml BSA and 0.05% sodium azide |
| Applications | Dilution |
| Western Blot (WB) | 1:2,000 |
| Product Specific Information |
| PA1-343 detects cytochrome P450 (P450) 3A4 from human and rat tissues and cells. This antibody detects, to a lesser extent, other P450 isoforms. PA1-343 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~46 kDa protein representing P450 3A4 from rat liver extracts. The PA1-343 immunogen is purified, His-tagged, full length human P450 3A4 fusion protein. Figure 1 illustrates Western blot detection of cytochrome P450 3A4 from rat liver extract using PA1-343. Figure 2 shows a Western blot of cytochrome P450 3A4 on rat liver extract using PA1-343. |
| General Information |
| The cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. |
| References: |
| Annu. Rev. Pharmacol. Toxicol., 39: 1-17, 1999. |
| Drug Metab. Dipos. 26(12): 1179-1184, 1998. |
(This product is for In Vitro experimental use only.)
 |
|||||||||||||
PO
Box 117, Rockford, IL 61105 USA |
 |
 |
