| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic peptide, corresponding to the C-terminal domain of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A. |
| Ordering Information | ||||
| Pierce Anti-MAFA DISCONTINUED Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-25994 | 50 µg | $0.00 | ||
| Storage: | 4º C, DO NOT FREEZE! |
| Form: | 50 µg of immunogen affinity purified antibody (1 mg/ml) in Tris citrate/phosphate with 0.1 % sodium azide; pH 7-8. |
| Applications | Dilution |
| ChIP assay (ChIP) | 5-15 µg for 10^8 cells |
| Gel Shift (GS) | 0.05 - 0.25 mg/ml |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000-1:3000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000-1:3000 |
| Western Blot (WB) | 1:1,000-1:10000 |
| Immunoelectrophoresis (IE) | 1-5 µg/20µl reaction |
| Product Specific Information |
| PA1-25994 detects MAFA from mouse samples. PA1-25994 has been successfully used in chromatin immunoprecipitation, immunoelectrophoresis, immunohistochemistry (frozen, paraffin), and Western blot procedures. The PA1-25994 immunogen is a synthetic peptide, corresponding to the C-terminal domain of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A. |
| General Information |
| Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and prodominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1 (-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development. |
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Box 117, Rockford, IL 61105 USA |
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