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PROTEASOME 19S SUBUNIT S12 ANTIBODIES > PROTEASOME 19S SUBUNIT S12 POLYCLONAL ANTIBODY PA1-1963
Anti-Proteasome 19S Subunit S12 Polyclonal Antibody
Printable Datasheet
Synonyms:
MOV34, P40, Rpn8, S12
Entrez Gene ID:
(Human) 5713, (Murine) 17463, (Rat) 307821
UniProt ID:
P51665
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Murine (Ms) Rat (Rt) Hamster (Hm) |
| Immunogen: | Synthetic peptide corresponding to residues A(55) V P F D E D D K D D S(66) C of human 19S subunit S12. |
| Ordering Information | ||||
| Pierce Anti-Proteasome 19S Subunit S12 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-1963 | 100 µg | $350.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of epitope affinity purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | 1 ug/ml |
| Product Specific Information |
| PA1-1963 detects proteasome 19S subunit S12 from human, rat, mouse and hamster cell lysates. PA1-1963 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~37 kDa protein representing proteasome 19S subunit S12 from human, rat, mouse, and hamster cell extracts. An additional unknown band is detected at ~48 kDa in some cell lysates. PA1-1963 immunogen is a synthetic peptide corresponding to residues A(55) V P F D E D D K D D S(66) C of human 19S subunit S12. The immunizing peptide sequence is 100% conserved in Danio rerio and 91% conserved in Drosphila, C. elegans and Anopheles. PA1-1963 immunizing peptide (Cat. # PEP-211) is available for use in neutralization and control experiments. Figure 1 shows a Western blot of proteasome 19S subunit S12 in K562 cell lysate using PA1-1963. Lane 1 is PA1-1963 Lane 2 is PA1-1963 pre-incubated with peptide |
| General Information |
| Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome. |
(This product is for In Vitro experimental use only.)
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