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Anti-ADFP Polyclonal Antibody

Printable Datasheet

Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Clone:
Label:
Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide corresponding to the internal region within residues 150-250 of mouse ADFP.
Applications Dilution
Western Blot (WB) 2 µg/ml
Ordering Information
Pierce Anti-ADFP Polyclonal Antibody
Product Number Pkg Size Price


PA1-16971 100 µl $285.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of peptide affinity purified rabbit antisera (0.98 mg/ml) in PBS with 30% glycerol.


Product Specific Information General Information
PA1-16971 detects ADFP from human samples.

PA1-16971 has been successfully used in Western blot applications. By Western blot this antibody detects a ~48 kDa band, representing ADFP.

The PA1-16971 immunogen is a synthetic peptide corresponding to the internal region within residues 150-250 of mouse ADFP.

Figure 1 illustrates Western blot detection of ADFP from human liver lysate using PA1-16971.
Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase.

ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
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References:

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