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Anti-DAF-3 Polyclonal Antibody

Synonyms:
Abnormal dauer formation protein 3, isoform c antibody.
Entrez Gene ID:
(C. elegans) 180431
UniProt ID:
O17532
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: C. elegans (Ce)
Immunogen: Synthetic peptide between amino acids 700-796 of the C.elegans DAF-3 protein.
Ordering Information
Pierce Anti-DAF-3 Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA1-16925 100 µl $285.00


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of whole antisera containing 0.05% sodium azide.


Applications Dilution
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:1000
Product Specific Information
PA1-16925 detects DAF-3 protein in C. elegans samples.

PA1-16925 has been successfully used in Western blot and immunoprecipitation procedures. PA1-16925 is specific for the ~91 kDa DAF-3 protein.

PA1-16925 immunogen is a synthetic peptide between amino acids 700-796 of the C.elegans DAF-3 protein.

Figure 1 shows a Western blot of wt c.elegans lysate showing specific labeling of the ~91 kDa DAF-3 protein.
General Information
Signals from TGF-beta superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans (C.elegans), defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. DAF-3 transgenes interfere with dauer formation, playing a role in the nucleus. DAF-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway.
(This product is for In Vitro experimental use only.)


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