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HEAT SHOCK FACTOR 1 ANTIBODIES > HEAT SHOCK FACTOR 1 POLYCLONAL ANTIBODY PA1-14203
Anti-Heat Shock Factor 1 Polyclonal Antibody
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Synonyms:
HSF1, HSTF1, heat shock factor 1
Entrez Gene ID:
(Human) 3297, (Murine) 15499
UniProt ID:
Q00613
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Murine (Ms) |
| Immunogen: | Synthetic peptide corresponding to human HSF1. |
| Ordering Information | ||||
| Pierce Anti-Heat Shock Factor 1 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-14203 | 200 µg | $298.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 200 µg of antibody (1 mg/ml) in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications | Dilution |
| Immunohistochemistry (IHC) | 1:50 |
| Product Specific Information |
| PA1-14203 detects HSF1 in human and mouse samples. PA1-14203 has been successfully used in immunohistochemistry procedures. The PA1-14203 immunogen is a synthetic peptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-S-P-P-R). |
| General Information |
| All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross X-reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes. |
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Immunohistochemistry with anti-Heat Shock Factor 1 Polyclonal Antibody (PA1-14203)
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Western Blot with anti-Heat Shock Factor 1 Polyclonal Antibody (PA1-14203)
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(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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