Anti-EDG1 Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) |
| Immunogen: |
Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. |
|
| Immunofluorescence (IF) |
5 ug/ml |
| Immunoprecipitation (IP) |
Assay dependent |
| Western Blot (WB) |
1 ug/ml |
| Pierce Anti-EDG1 Polyclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
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| PA1-1040 |
100 µg |
$354.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 ug of epitope affinity purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
PA1-1040 detects S1P1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
PA1-1040 has successfully been used in immuno-fluorescence, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040.
The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments.
Figure 1 illustrates immunofluorescence staining of S1P1 in transfected CCL39 cells using PA1-1040.
Figure 2 illustrates Western blot detection of S1P1 from transfected HEK293 cell lysate using PA1-1040. |
Sphingosine 1-phosphate (S1P) is bioactive lipid derived from the metabolism of sphingomyelin. S1P is predominately synthesized, stored, and released from platelets. When released, this molecule stimulates signaling pathways via G-protein coupled receptors. One such receptor is the heterotrimeric guanine nucleotide binding protein shingosine 1-phosphate receptor type 1 (S1P1,also known as EDG1).
Research has demonstrated that S1P regulates numerous cellular responses including, wound healing, cell growth, prevention of apoptosis, vascular maturation and cellular migration and survival. In studies involving S1P1 deficient embryos, blood vessels where incompletely maturated with smooth muscles cells, resulting in embryonic death. In contrast, a separate study demonstrated that overexpression of S1P1 led to the stimulation of cell migration. |
 
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No references for this product. |
This product is for In Vitro experimental use only
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