Anti-RANBP2 Polyclonal Antibody
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Synonyms:
Ran Binding Protein 2, RBP2, Nuclear pore complex protein Nup358, Nucleoporin Nup358, 358 kDa nucleoporin, P270
Entrez Gene ID:
(Human) 5903
UniProt ID:
P49792
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Bovine (Bv) |
| Immunogen: | Synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2. |
| Ordering Information | ||||
| Pierce Anti-RANBP2 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-082 | 100 µg | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Immunohistochemistry (IHC) | 10 µg/ml |
| Western Blot (WB) | 1 µg/ml |
| Product Specific Information |
| PA1-082 detects Ran binding protein 2 (RanBP2) from human and bovine retina. PA1-082 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~360 kDa protein representing RanBP2 from bovine retinal extracts. Immunohistochemical staining of bovine retina with PA1-082 results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells. The PA1-082 immunogen is a synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2. This sequence is completely conserved between human and mouse. The PA1-082 immunizing peptide (Cat. # PEP-137) is available for use in neutralization and control experiments. Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using PA1-082. Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using PA1-082. |
| General Information |
| Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane. |
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Immunohistochemistry with anti-RANBP2 Polyclonal Antibody (PA1-082)
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| References: |
| Mamm. Genome, 12(6):406-15, 2001. |
| Methods Enzymol. 315:455-68, 2000. |
| Trends Cell Biol. 9(8):310-1, 1999. |
(This product is for In Vitro experimental use only.)
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