Anti-alpha COP Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) Rat (Rt) |
| Immunogen: |
Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP. |
|
| Immunocytochemistry (ICC) |
3 µg/ml |
| Western Blot (WB) |
1 µg/ml |
| Pierce Anti-alpha COP Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
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| PA1-067 |
100 µg |
$350.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
PA1-067 detects coatomer-protein I alpha (COPI alpha) from human and rat cells.
PA1-067 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing COP alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha-COP using PA1-067 results in alpha-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.
PA1-067 immunogen is a synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP. The PA1-067 immunizing peptide (Cat. # PEP-125) is available for use in neutralization and control experiments.
Figure 1 illustrates immunolocalization of COP alpha in HeLa cells using PA1-067. |
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. |
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This product is for In Vitro experimental use only
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