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EARLY ENDOSOMAL ANTIGEN 1 ANTIBODIES > EARLY ENDOSOMAL ANTIGEN 1 POLYCLONAL ANTIBODY PA1-063A
Anti-Early Endosomal Antigen 1 Polyclonal Antibody
Printable Datasheet
Synonyms:
EEA1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Entrez Gene ID:
(Murine) 216238
UniProt ID:
Q8BL66
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic peptide corresponding to residues T(1391) P S S K K P V R V C D A C F N D L Q G(1410) of human EEA1. |
| Ordering Information | ||||
| Pierce Anti-Early Endosomal Antigen 1 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-063A | 100 µg | $357.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | Assay dependent |
| Product Specific Information |
| PA1-063A detects early endosomal antigen 1 (EEA1) from mouse samples. Previous lots of this antibody have been used on human, hamster, canine, and rat samples. PA1-063A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~160 kDa protein representing EEA1 from mouse brain extract. Previous lots of this antibody have been used in immunoprecipitation and immunofluorescence procedures. The PA1-063A immunogen is a synthetic peptide corresponding to residues T(1391) P S S K K P V R V C D A C F N D L Q G(1410) of human EEA1. The PA1-063A immunizing peptide (Cat. # PEP-079) is available for use in neutralization and control experiments. Figure 1 illustrates Western blot detection of EEA1 from mouse brain using PA1-063A. Lane 2 shows incubation with the neutralizing peptide (PEP-079). |
| General Information |
| Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. |
(This product is for In Vitro experimental use only.)
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