| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Murine (Ms) Rat (Rt) |
| Immunogen: | Synthetic peptide corresponding to residues M(1) N F L R G V M G G Q S A G P Q H(17) of rat p115. |
| Ordering Information | ||||
| Pierce Anti-p115 Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| PA1-006 | 100 µl | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of peptide affinity purified rabbit IgG (1mg/ml) in PBS containing 1mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | 1:500 |
| Product Specific Information |
| PA1-006 detects p115 in rat and mouse samples. PA1-006 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~115 kDa protein representing p115 in rat and mouse liver samples. The PA1-006 immunogen is a synthetic peptide corresponding to residues M(1) N F L R G V M G G Q S A G P Q H(17) of rat p115. This sequence is conserved across many species including mouse and human. PA1-006 immunizing peptide (Cat. # PEP-276) is available for use in neutralization and control experiments. Figure 1 illustrates Western blot detection of p115 in rat liver using PA1-006. |
| General Information |
| p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments. |
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