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FLIP DISCONTINUED ANTIBODIES > FLIP DISCONTINUED POLYCLONAL ANTIBODY OPA1-01011
Anti-FLIP DISCONTINUED Polyclonal Antibody
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Synonyms:
Casper, Inhibitor of FLICE, I-FLICE, FLAME-1,CASH, CLARP, c-FLIP, MRIT, CASH, Usurpin
Entrez Gene ID:
(Human) 8837
UniProt ID:
O15519
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic Peptide: C N(447) G Y M Y D W N S R V S A K E K Y Y(464) |
| Ordering Information | ||||
| Pierce Anti-FLIP DISCONTINUED Polyclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| OPA1-01011 | 100 µg | $339.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of purified IgG (0.5 mg/ml) in 200 µl of PBS containing 0.02% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | 1:1,000 |
| Product Specific Information |
| OPA1-01011 detects the caspases-8 (FLICE (FADD (fas-associated via death domain) -like ICE) ) inhibitory protein (FLIP) from human cells. This antibody shows no cross-reactivity with the short form of FLIP, FLIPS. OPA1-01011 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~55 kDa protein representing the long form of FLIP, FLIPL, in HeLa cell lysate. OPA1-01011 immunizing peptide corresponds to amino acid residues 447-464 from human FLIP. OPA1-01011 immunizing peptide (Cat. # OPEP-01011) is available for use in neutralization and control experiments. Figure 1 shows a Western blot of FLIP in HeLa cell lysate using OPA1-01011. |
| General Information |
| Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. Caspases-8 (FLICE (FADD (fas-associated via death domain) -like ICE)) and -10 (FLICE2 (FADD (fas-associated via death domain) -like ICE 2)) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins (FLIPs) were identified in virus and humans and designated v-FLIPs and FLIP, respectively. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein ADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1). |
| References: |
| JBC Vol. 279 No. 19, 20221-20233, Jun 2004 |
| Nature, 388: 190-195, 1997. |
| Nature, 386: 517-521, 1997. |
(This product is for In Vitro experimental use only.)
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