Anti-FLIP DISCONTINUED Polyclonal Antibody
| Host / Isotype: |
Rabbit |
| Class: |
Polyclonal |
| Type: |
Antibody |
| Clone: |
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| Label: |
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| Species Reactivity: |
Human (Hu) |
| Immunogen: |
Synthetic Peptide: C N(447) G Y M Y D W N S R V S A K E K Y Y(464) |
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| Western Blot (WB) |
1:1,000 |
| Pierce Anti-FLIP DISCONTINUED Polyclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
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| OPA1-01011 |
100 µg |
$335.87 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 ug of purified IgG (0.5 mg/ml) in 200 µl of PBS containing 0.02% sodium azide. |
OPA1-01011 detects the caspases-8 (FLICE (FADD (fas-associated via death domain) -like ICE) ) inhibitory protein (FLIP) from human cells. This antibody shows no cross-reactivity with the short form of FLIP, FLIPS.
OPA1-01011 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~55 kDa protein representing the long form of FLIP, FLIPL, in HeLa cell lysate.
OPA1-01011 immunizing peptide corresponds to amino acid residues 447-464 from human FLIP. OPA1-01011 immunizing peptide (Cat. # OPEP-01011) is available for use in neutralization and control experiments.
Figure 1 shows a Western blot of FLIP in HeLa cell lysate using OPA1-01011. |
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. Caspases-8 (FLICE (FADD (fas-associated via death domain) -like ICE)) and -10 (FLICE2 (FADD (fas-associated via death domain) -like ICE 2)) are two pivotal members in the ICE/CED-3 protease family.
FLICE-inhibitory proteins (FLIPs) were identified in virus and humans and designated v-FLIPs and FLIP, respectively. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein ADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1). |
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This product is for In Vitro experimental use only
Printable Datasheet
