Part of Thermo Fisher Scientific

Anti-FLIP DISCONTINUED Polyclonal Antibody

Synonyms:
Casper, Inhibitor of FLICE, I-FLICE, FLAME-1,CASH, CLARP, c-FLIP, MRIT, CASH, Usurpin
Entrez Gene ID:
(Human) 8837
UniProt ID:
O15519
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Human (Hu)
Immunogen: Synthetic Peptide: C N(447) G Y M Y D W N S R V S A K E K Y Y(464)
Ordering Information
Pierce Anti-FLIP DISCONTINUED Polyclonal Antibody
Product Number Pkg. Size Price Purchase
OPA1-01011 100 µg $339.00  


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ug of purified IgG (0.5 mg/ml) in 200 µl of PBS containing 0.02% sodium azide.


Applications Dilution
Western Blot (WB) 1:1,000
Product Specific Information
OPA1-01011 detects the caspases-8 (FLICE (FADD (fas-associated via death domain) -like ICE) ) inhibitory protein (FLIP) from human cells. This antibody shows no cross-reactivity with the short form of FLIP, FLIPS.

OPA1-01011 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~55 kDa protein representing the long form of FLIP, FLIPL, in HeLa cell lysate.

OPA1-01011 immunizing peptide corresponds to amino acid residues 447-464 from human FLIP. OPA1-01011 immunizing peptide (Cat. # OPEP-01011) is available for use in neutralization and control experiments.

Figure 1 shows a Western blot of FLIP in HeLa cell lysate using OPA1-01011.
General Information
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. Caspases-8 (FLICE (FADD (fas-associated via death domain) -like ICE)) and -10 (FLICE2 (FADD (fas-associated via death domain) -like ICE 2)) are two pivotal members in the ICE/CED-3 protease family.

FLICE-inhibitory proteins (FLIPs) were identified in virus and humans and designated v-FLIPs and FLIP, respectively. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein ADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1).
References:
JBC Vol. 279 No. 19, 20221-20233, Jun 2004
Nature, 388: 190-195, 1997.
Nature, 386: 517-521, 1997.
(This product is for In Vitro experimental use only.)


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