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BROMODEOXYURIDINE ANTIBODIES > BROMODEOXYURIDINE MONOCLONAL ANTIBODY OMA1-06200
Anti-Bromodeoxyuridine Monoclonal Antibody
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Synonyms:
BrdU
| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | IIB5 |
| Species Reactivity: | Chemical (Chem) |
| Immunogen: | Purified bromodeoxyuridine. |
| Ordering Information | ||||
| Pierce Anti-Bromodeoxyuridine Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| OMA1-06200 | 100 µg | $337.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of purified antibody (1 mg/ml) in PBS containing 0.09% sodium azide. |
| Applications | Dilution |
| Flow Cytometry (FACS) | 1:100 - 1:200 |
| Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) | 1:100 - 1:200 |
| Product Specific Information |
| OMA1-06200 detects bromodeoxyuridine (BrdU). OMA1-06200 has been successfully used in immunohistochemical and FACS procedures. This antibody specifically detects free BrdU and BrdU that has been incorporated into nuclear DNA. OMA1-06200 antigen is purified BrdU. |
| General Information |
| The life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase. The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthesized DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. |
| References: |
| Cytometry, 8: 372-376, 1987. |
(This product is for In Vitro experimental use only.)
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