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Anti-NMDA Receptor 1 Monoclonal Antibody

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Details
Host / Isotype: Mouse
Class: Monoclonal
Type: Antibody
Clone: R1JHL
Label:
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt)
Immunogen: Fusion protein corresponding to amino acids 1-564 of rat NMDA NR1.
Applications Dilution
Immunoprecipitation (IP) 3 µg/200µg lysate
Western Blot (WB) 0.3 µg/ml
Ordering Information
Pierce Anti-NMDA Receptor 1 Monoclonal Antibody
Product Number Pkg Size Price


OMA1-04010 15 µg $339.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 15 µg of lyophilized affinity purified antibody. The antibody should be reconstituted in 50µl of phosphate buffered saline (137mM NaCl, 7.5mM Na2HPO4, 2.7mM KCl, 1.5mM KH2PO4, pH 7.4) before use.


Product Specific Information General Information
OMA1-04010 detects N-methyl-D-aspartate (NMDA) receptor type 1 subunit (NR1) from human, mouse, and rat tissues. This antibody shows no cross-reactivity with any of the NR2 subunits.

OMA1-04010 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~120 kDa protein representing NR1 in rat brain homogenate.

OMA1-04010 antigen is a fusion protein corresponding to amino acids 1-564 from rat NR1.
N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity.

A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time.
References:
Mol. Pharm., 51: 79-86, 1997.
J.Neurochem., 65:176-183, 1995
J. Neuroscience 24(38), 8253-8264, Sept. 2004
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