Anti-NMDA Receptor 1 Monoclonal Antibody
| Host / Isotype: |
Mouse |
| Class: |
Monoclonal |
| Type: |
Antibody |
| Clone: |
R1JHL |
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: |
Fusion protein corresponding to amino acids 1-564 of rat NMDA NR1. |
|
| Immunoprecipitation (IP) |
3 µg/200µg lysate |
| Western Blot (WB) |
0.3 µg/ml |
| Pierce Anti-NMDA Receptor 1 Monoclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
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| OMA1-04010 |
15 µg |
$339.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
15 µg of lyophilized affinity purified antibody. The antibody should be reconstituted in 50µl of phosphate buffered saline (137mM NaCl, 7.5mM Na2HPO4, 2.7mM KCl, 1.5mM KH2PO4, pH 7.4) before use. |
OMA1-04010 detects N-methyl-D-aspartate (NMDA) receptor type 1 subunit (NR1) from human, mouse, and rat tissues. This antibody shows no cross-reactivity with any of the NR2 subunits.
OMA1-04010 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~120 kDa protein representing NR1 in rat brain homogenate.
OMA1-04010 antigen is a fusion protein corresponding to amino acids 1-564 from rat NR1. |
N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity.
A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time. |
This product is for In Vitro experimental use only
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