| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | mAbP2-1 |
| Species Reactivity: | Human (Hu) Non-human primate (Nhp) |
| Immunogen: | Native, secreted form of APP |
| Ordering Information | ||||
| Pierce Anti-Amyloid Precursor Protein Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| OMA1-03132 | 50 µg | $312.00 | ||
| Storage: | -80º C, Avoid Freeze/Thaw Cycles |
| Form: | 50 ug of Protein G purified antibody in PBS, pH 7.4. |
| Applications | Dilution |
| ELISA (ELISA) | 10 µg/ml |
| Immunocytochemistry (ICC) | 0.5-5 µg/ml |
| Immunoprecipitation (IP) | 25 µg/ml |
| Western Blot (WB) | 5 µg/ml |
| Product Specific Information |
| OMA1-03132 detects amyloid precursor protein (APP) from human and non-human primate tissues. This antibody is specific for native, non-denatured protein, and does not cross-react with mouse, rat APP or other APP homologs. OMA1-03132 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation and ELISA procedures. This antibody does not work in in IHC on paraffin embedded tissues nor does it recognize APP if the samples are boiled in Laemmli (SDS) sample uffer conatining a reducking agent such as DTT or ß-ME. OMA1-03132 antigen is the native, secreted form of human APP. The epitope recognized by OMA1-03132 maps to residues 104-118 of APP |
| General Information |
| Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. |
| References: |
| JBC Vol. 280 No.13, 12523-12535, Apr 2005 |
| Nature, 341:546-549, 1989. |
| Am. J. Pathol., 142:1449-1457, 1993. |
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