Anti-Phospho-AKT (Ser473) Monoclonal Antibody
| Host / Isotype: |
Rabbit / IgG |
| Class: |
Monoclonal |
| Type: |
Antibody |
| Clone: |
14-6 |
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) |
| Immunogen: |
Synthetic phosphopeptide derived from a region of human PKB that contains serine 473. |
|
| Immunocytochemistry (ICC) |
1:100 |
| Western Blot (WB) |
1:1000 |
| Pierce Anti-Phospho-AKT (Ser473) Monoclonal Antibody |
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| Product Number |
Pkg Size |
Price |
|
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| OMA1-03061 |
100 µl |
$352.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µl of IgG in Dulbecco's PBS (without Mg2+ and Ca+), pH 7.3, 50% glycerol, 1.0 mg/mL BSA, 0.05% sodium azide. Volume is lot dependent based on activity, and each vial contains material for 10 blots. |
OMA1-03061 detects protein kinase B (PKB) phosphorylated at Ser473 from human and mouse samples. OMA1-03061 detects PKB 1,2,and 3.
OMA1-03061 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing phosphorylated PKB (Ser473) from NIH-3T3 cells treated with platelet-derived growth factor (PDGF).
The OMA1-03061 immunizing phosphopeptide was derived from a region of human PKB that contains serine 473. There is 100% homology with other species, that have not yet been tested. |
Protein kinase B (PKB), also known as Akt, is an oncogenic serine-threonine kinase originally cloned from the retrovirus AKT8. PKB is a direct target of phosphatidylinositol 3-kinase (PI3-kinase) and is activated in response to a variety of growth factors including insulin and platelet-derived growth factor (PDGF). PKB also plays an important role in many PI3-kinase dependent cellular functions including inhibition of apoptosis.
Activity of PKB has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to PKB activity, however phosphorylation of T308 has been shown to be absolutely essential for PKB activation. |
No references for this product. |
This product is for In Vitro experimental use only
Printable Datasheet
