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SODIUM/POTASSIUM ATPASE ALPHA-1 ANTIBODIES > SODIUM/POTASSIUM ATPASE ALPHA-1 MONOCLONAL ANTIBODY MA3-929
Anti-Sodium/Potassium ATPase alpha-1 Monoclonal Antibody
Printable Datasheet
Synonyms:
Na+/K+ ATPase Alpha-1
Entrez Gene ID:
(Human) 476
UniProt ID:
P05023
| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | M8-P1-A3 |
| Species Reactivity: | Human (Hu) Rat (Rt) Canine (Ca) Ovine (Ov) Porcine (Po) |
| Immunogen: | Lamb kidney alpha 1 sodium/potassium ATPase. |
| Ordering Information | ||||
| Pierce Anti-Sodium/Potassium ATPase alpha-1 Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA3-929 | 200 µl | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 200 µl of PBS-diluted ascites containing 0.05% sodium azide. |
| Applications | Dilution |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
| Western Blot (WB) | 1:250 |
| Product Specific Information |
| MA3-929 detects sodium/potassium ATPase alpha 1 in sheep, canine, rat, human and porcine tissues. This antibody does not detect the alpha 1 isoform in Xenopus and chicken. MA3-929 does not detect the alpha 2 or alpha 3 isoform in rat. MA3-929 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha 1 sodium/potassium ATPase from canine kidney extract. Immunohistochemical staining of sodium/potassium ATPase alpha 1 with MA3-929 yields a pattern consistent with plasma membrane localization. The MA3-929 antigen is sheep kidney alpha 1 sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase. |
| General Information |
| The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle. |
(This product is for In Vitro experimental use only.)
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