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Anti-PDI Monoclonal Antibody

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Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: RL77
Label:
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Hamster (Hm) Porcine (Po) Xenopus laevis (Xl)
Immunogen: Purified rat PDI protein.
Applications Dilution
Flow Cytometry (FACS) Assay dependent
Immunohistochemistry (IHC) 1:100
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:1,000
Ordering Information
Pierce Anti-PDI Monoclonal Antibody
Product Number Pkg Size Price


MA3-018 100 µl $361.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of ascites fluid (2.3 mg/ml) with 0.05% sodium azide.


Product Specific Information General Information
MA3-018 detects protein disulphide-isomerase (PDI) from human, mouse, rat, porcine, xenopus liver tissues as well as hamster (CHO) cells.

MA3-018 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a 59 kDa protein representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-018 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-018 has been shown to inhibit the activity of PDI in vitro.

In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.

The MA3-018 antigen is purified rat PDI.

Figure 1 shows immunolocalization of PDI in rat epithelial cells using MA3-018.
The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
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References:
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JBC 277(35):32369-32378, 2002.
Mol. Endo. 2006 manuscript, me.2006-006v1.
J. Am. Soc. Nephrol., Jan 2001; 12: 1.
Mol. Biol. Cell, May 2000; 11: 1509.
J. Immunol., Apr 2000; 164: 4120.
Mol. Biol. Cell, Sep 2006; 17: 3781 - 3792.
J Am Soc Nephrol 12:1-9, 2001
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