Anti-Galectin 3 Monoclonal Antibody
| Host / Isotype: |
Mouse / IgG1 |
| Class: |
Monoclonal |
| Type: |
Antibody |
| Clone: |
A3A12 |
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: |
Full length recombinant human galectin-3. |
|
| ELISA (ELISA) |
Assay dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) |
6 µg/ml |
| Western Blot (WB) |
1 µg/ml |
| Pierce Anti-Galectin 3 Monoclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
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| MA1-940 |
100 µg |
$354.00 |
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| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µg of purified IgG1 (1 mg/ml) in PBS containing 0.05% sodium azide. |
MA1-940 detects galectin-3 from human, mouse and rat tissues.
MA1-940 has been successfully used in Western blot, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~30 kDa protein representing galectin-3 from Jurkat cells transfected with human galectin-3. Immunohistochemical staining of galectin-3 in rat olfactory bulb with MA1-940 yields a pattern consistent with nuclear and plasma membrane staining.
MA1-940 antigen is full length recombinant human galectin-3. The epitope for this antibody has been mapped to the first 58 amino acids of galectin-3.
Figure 1 shows immunolocalization of galectin-3 in rat olfactory bulb using MA1-940. |
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Galectin-3 is one of the more extensively studied member of this family and is a 30 kDa protein known by various other names including eBP (IgE binding protein), Mac-2, CBP-30 and L-29. Due to a C-terminal carbohydrate binding site, galectin-3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. MA1-940 has been shown to potentiate the binding of galectin-3 to IgG.
Among its numerous proposed functions, galectin-3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion.
One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin-3 shares an NWGR motif with Bcl-2 which has been shown to be an important region in Bcl-2 for suppression of apoptosis. Galectin-3 has been shown to interact with Bcl-2 which may account for the ability of galectin-3 to suppress programmed cell death in anti-Fas (an apoptosis inducer) treated Jurkat cells transfected with galectin-3. In contrast to galectin-3, galectin-1 actually induces apoptosis when added to cultures of activated human T cells. |
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This product is for In Vitro experimental use only
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