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GALECTIN 3 ANTIBODIES > GALECTIN 3 MONOCLONAL ANTIBODY MA1-940
Anti-Galectin 3 Monoclonal Antibody
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Synonyms:
BP (IgE Binding Protein), Mac-2, CBP-30, L-29
Entrez Gene ID:
(Human) 3958, (Murine) 16854, (Rat) 83781
UniProt ID:
P17931
| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | A3A12 |
| Species Reactivity: | Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: | Full length recombinant human galectin-3. |
| Ordering Information | ||||
| Pierce Anti-Galectin 3 Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-940 | 100 µg | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of purified IgG1 (1 mg/ml) in PBS containing 0.05% sodium azide. |
| Applications | Dilution |
| ELISA (ELISA) | Assay dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 6 µg/ml |
| Western Blot (WB) | 1 µg/ml |
| Product Specific Information |
| MA1-940 detects galectin-3 from human, mouse and rat tissues. MA1-940 has been successfully used in Western blot, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~30 kDa protein representing galectin-3 from Jurkat cells transfected with human galectin-3. Immunohistochemical staining of galectin-3 in rat olfactory bulb with MA1-940 yields a pattern consistent with nuclear and plasma membrane staining. MA1-940 antigen is full length recombinant human galectin-3. The epitope for this antibody has been mapped to the first 58 amino acids of galectin-3. Figure 1 shows immunolocalization of galectin-3 in rat olfactory bulb using MA1-940. |
| General Information |
| Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Galectin-3 is one of the more extensively studied member of this family and is a 30 kDa protein known by various other names including eBP (IgE binding protein), Mac-2, CBP-30 and L-29. Due to a C-terminal carbohydrate binding site, galectin-3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. MA1-940 has been shown to potentiate the binding of galectin-3 to IgG. Among its numerous proposed functions, galectin-3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin-3 shares an NWGR motif with Bcl-2 which has been shown to be an important region in Bcl-2 for suppression of apoptosis. Galectin-3 has been shown to interact with Bcl-2 which may account for the ability of galectin-3 to suppress programmed cell death in anti-Fas (an apoptosis inducer) treated Jurkat cells transfected with galectin-3. In contrast to galectin-3, galectin-1 actually induces apoptosis when added to cultures of activated human T cells. |
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Immunofluorescence with anti-Galectin 3 Monoclonal Antibody [A3A12] (MA1-940)
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(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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