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C/EBP BETA ANTIBODIES > C/EBP BETA MONOCLONAL ANTIBODY MA1-827
Anti-C/EBP beta Monoclonal Antibody
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Synonyms:
CCAAT/Enhancer Binding Protein Beta
Entrez Gene ID:
(Murine) 12608, (Rat) 24253
UniProt ID:
P28033
| Details | |
| Host / Isotype: | Mouse / IgG |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 1H7 |
| Species Reactivity: | Murine (Ms) Rat (Rt) |
| Immunogen: | Recombinant mouse LIP. |
| Ordering Information | ||||
| Pierce Anti-C/EBP beta Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-827 | 100 µg | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of protein G purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Immunofluorescence (IF) | 4 ug/ml |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1 ug/ml |
| Product Specific Information |
| MA1-827 detects C/EBP beta protein in mouse and rat samples. MA1-827 detects the LAP*, LAP and LIP isoforms of C/EBP beta. MA1-827 has successfully been used in Western blot, immunofluorescent and immunoprecipitation procedures. By Western blot, this antibody detects 38, 35 and 20 kDa proteins representing C/EBP beta LAP*, LAP, and LIP isoforms respectively, in 3T3-L1 cells. LIP is recognized as the C-terminus fragment. Immunofluorescent staining of C/EBP beta in P388D1 cells can be performed using MA1-827 The MA1-827 immunogen is recombinant mouse LIP. Figure 1 illustrates Western blot of the 3T3-L1 cell lysate using MA1-827. Figure 2 illustrates immunofluorescence staining of C/EBP beta in P388D1 cells using MA1-827. |
| General Information |
| The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients. |
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Immunofluorescence with anti-C/EBP beta Monoclonal Antibody [1H7] (MA1-827)
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Western Blot with anti-C/EBP beta Monoclonal Antibody [1H7] (MA1-827)
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| References: |
| JBC, 278(51): 51150-51158, 2003. |
(This product is for In Vitro experimental use only.)
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