Anti-C/EBP beta Monoclonal Antibody
| Host / Isotype: |
Mouse / IgG1 |
| Class: |
Monoclonal |
| Type: |
Antibody |
| Clone: |
18F8 |
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: |
Synthetic peptide corresponding to residues M(1) H R L L A W D A A C L P P P A A F P K L H(21) of mouse C/EBP beta. |
|
| Immunoprecipitation (IP) |
Assay dependent |
| Western Blot (WB) |
2 ug/ml |
| Pierce Anti-C/EBP beta Monoclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
|
| MA1-826 |
100 µg |
$350.00 |
|
|
|
|
| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 ug of protein G purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
MA1-826 detects C/EBP beta protein in human, mouse, and rat samples. MA1-826 only detects the LAP* isoform of C/EBP beta.
MA1-826 has successfully been used in Western blot, immunofluorescent and immunoprecipitation procedures. By Western blot, this antibody detects a 37 kDa protein representing LAP* of C/EBP beta (N-terminus fragment) in transfected 293T cells.
The MA1-826 immunogen is a synthetic peptide corresponding to residues M(1) H R L L A W D A A C L P P P A A F P K L H(21) of mouse C/EBP beta. This peptide (Cat. # PEP-243) is available for use in neutralization and control experiments.
Figure 1 illustrates Western blot detection of the LAP* on transfected C/EBP beta using MA1-826.
Figure 2 illustrates immunofluorescence staining of C/EBP beta in P388D1 cells using MA1-826. |
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients. |
 
|
This product is for In Vitro experimental use only
Printable Datasheet
