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Anti-C/EBP beta Monoclonal Antibody

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Synonyms:
CCAAT/Enhancer Binding Protein Beta
Entrez Gene ID:
(Human) 1051, (Murine) 12608, (Rat) 24253
UniProt ID:
P17676
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 18F8
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues M(1) H R L L A W D A A C L P P P A A F P K L H(21) of mouse C/EBP beta.
Ordering Information
Pierce Anti-C/EBP beta Monoclonal Antibody
Product Number Pkg. Size Price Purchase
MA1-826 100 µg $350.00


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ug of protein G purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 2 ug/ml
Product Specific Information
MA1-826 detects C/EBP beta protein in human, mouse, and rat samples. MA1-826 only detects the LAP* isoform of C/EBP beta.

MA1-826 has successfully been used in Western blot, immunofluorescent and immunoprecipitation procedures. By Western blot, this antibody detects a 37 kDa protein representing LAP* of C/EBP beta (N-terminus fragment) in transfected 293T cells.

The MA1-826 immunogen is a synthetic peptide corresponding to residues M(1) H R L L A W D A A C L P P P A A F P K L H(21) of mouse C/EBP beta. This peptide (Cat. # PEP-243) is available for use in neutralization and control experiments.

Figure 1 illustrates Western blot detection of the LAP* on transfected C/EBP beta using MA1-826.

Figure 2 illustrates immunofluorescence staining of C/EBP beta in P388D1 cells using MA1-826.
General Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues.  These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP.  One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells.  Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation.  From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
Immunofluorescence with anti-C/EBP beta Monoclonal Antibody [18F8] (MA1-826)
References:
JBC, 278(51): 51150-51158, 2003.
(This product is for In Vitro experimental use only.)


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