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ARYL HYDROCARBON RECEPTOR ANTIBODIES > ARYL HYDROCARBON RECEPTOR MONOCLONAL ANTIBODY MA1-514
Anti-Aryl Hydrocarbon Receptor Monoclonal Antibody
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Synonyms:
ARNT, Aryl Hydrocarbon Receptor Nuclear Translocator, Dioxin Receptor, Hypoxia Inducible Factor 1, Tango, HIF1 beta
Entrez Gene ID:
(Human) 196, (Murine) 11622, (Rat) 25690
UniProt ID:
P35869
| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | RPT1 |
| Species Reactivity: | Human (Hu) Murine (Ms) Rat (Rt) |
| Immunogen: | Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR. |
| Ordering Information | ||||
| Pierce Anti-Aryl Hydrocarbon Receptor Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-514 | 100 µl | $358.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of ascites containing 0.05% sodium azide. |
| Applications | Dilution |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay dependent |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1:1,000 |
| Product Specific Information |
| MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells. MA1-514 has been successfully used in Western blotting, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-514 can be used in immunoprecipitation procedures, MA1-513 (clone RPT9) is recommended for this procedure. MA1-514 immunogen is residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted. |
| General Information |
| The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor. Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1. |
(This product is for In Vitro experimental use only.)
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