MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-514 has been successfully used in Western blotting, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-514 can be used in immunoprecipitation procedures, MA1-513 (clone RPT9) is recommended for this procedure.
MA1-514 immunogen is residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted. |
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1. |
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