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Anti-Aryl Hydrocarbon Receptor Monoclonal Antibody

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Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: RPT1
Label:
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
Applications Dilution
Immunohistochemistry (Paraffin) (IHC (P)) Assay dependent
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:1,000
Ordering Information
Pierce Anti-Aryl Hydrocarbon Receptor Monoclonal Antibody
Product Number Pkg Size Price


MA1-514 100 µl $358.00
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of ascites containing 0.05% sodium azide.


Product Specific Information General Information
MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.

MA1-514 has been successfully used in Western blotting, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-514 can be used in immunoprecipitation procedures, MA1-513 (clone RPT9) is recommended for this procedure.

MA1-514 immunogen is residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted.
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
 
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
References:
Toxicology, 153(1-3):73-82, 2000.
Hybridoma, 14:279-283, 1995.
J. Biol. Chem., 269(44):27544-27558, 1994.
Hybridoma, 14(3):279-283, 1995
Archives of Biochem and Biophys. 406:209-221, 2002
Mol. Pharmacology, Vol. 65, No. 1, 165-171 Jan 2004
JBC Vol. 279 No. 26, 27187-27193, Jun 2004
J. Bio. Chem:276(5);3054-3059, 2001
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This product is for In Vitro experimental use only
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