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FAS LIGAND ANTIBODIES > FAS LIGAND MONOCLONAL ANTIBODY MA1-4804
Anti-Fas Ligand Monoclonal Antibody
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Synonyms:
FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1
Entrez Gene ID:
(Human) 355
UniProt ID:
P25445
| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 2 |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide corresponding to residues 157-343 of human Fas Ligand. |
| Ordering Information | ||||
| Pierce Anti-Fas Ligand Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-4804 | 100 µg | $434.00 | ||
| Storage: | 4º C |
| Form: | 100 µg of protein A/G purified mouse IgG1 (0.1mg/ml) in 1 ml PBS with 1% BSA, and 0.05% sodium azide, pH 7.4. |
| Applications | Dilution |
| Immunofluorescence (IF) | 1:100 to 1:1000 |
| Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) | 1:10 to 1:100 |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1:100 to 1:1000 |
| Product Specific Information |
| MA1-4804 detects Fas Ligand (FasL, CD95L) from human samples. MA1-4804 has been successfully used in immunohistochemistry (paraffin and frozen), immunofluorescence, immunoprecipitation and Western blot procedures. Negative after pre-adsorption with FasL-peptide for Western blot and paraffin sections. By Western blot, this antibody detects a ~45 kDa protein representing the membrane bound form of FasL in human T-lymphocyte, NK, testis, eye, thymus, liver, ovary, lung, heart, and kidney samples. The MA1-4804 immunogen is a synthetic peptide corresponding to residues 157-343 of human Fas Ligand. Figure 1 illustrates immunohistochemical staining of FasL in paraffin embedded human stomachcarcinoma tissues using MA1-4804. |
| General Information |
| Fas ligand (FasL, CD 95L) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas (CD 95, Apo-1) is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family FasL induces apoptosis when interacting with its receptor Fas. FasL may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of FasL to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. FasL is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. FasL expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. The Fas/FasL system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer. It is assumed that induction of apoptosis through FasL is predominantly involved in anti-viral immune responses. |
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Immunohistochemistry with anti-Fas Ligand Monoclonal Antibody [2] (MA1-4804)
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(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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