Anti-APE1 Monoclonal Antibody
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Synonyms:
Apurinic/Apyrimidinic Endonuclease
Entrez Gene ID:
(Human) 328
UniProt ID:
P27695
| Details | |
| Host / Isotype: | Mouse / IgG2a |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 13B 8E5C2 |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Full-length recombinant human APE protein. |
| Ordering Information | ||||
| Pierce Anti-APE1 Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-440 | 100 µl | $347.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl purified antibody (1 mg/ml) in PBS containing 0.05% sodium azide. |
| Applications | Dilution |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1:1,000 |
| Product Specific Information |
| MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human tissues. MA1-440 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 37 kDa protein representing APE/ref-1 in HeLa cell lysate. Immunohistochemical staining of APE/ref-1 in a variety of normal and cancerous human tissues, including ovaries, cervix, prostate, germ cell tumors, osteosarcomas, and rhabdosarcomas, with MA1-440 yields a staining pattern consistent with nuclear staining. The MA1-440 immunogen is full-length, recombinant human APE protein. Figure 1 shows immunolocalization of APE/ref-1 in prostatic carcinoma using MA1-440. |
| General Information |
| Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis. |
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Immunohistochemistry with anti-APE1 Monoclonal Antibody [13B 8E5C2] (MA1-440)
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| References: |
| Clin. Cancer Res., Vol 9: 4689-4694, Oct 2003 |
| Cell Growth and Diff., 8: 443-449, 1997. |
| Cancer Res., 362: 6097-6102, 1995. |
| EMBO, 11: 653-665, 1992. |
(This product is for In Vitro experimental use only.)
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Box 117, Rockford, IL 61105 USA |
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