Anti-DAP3 Monoclonal Antibody
Printable Datasheet
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Synonyms:
DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10
Entrez Gene ID:
(Human) 7818, (Murine) 65111
UniProt ID:
P51398
| Details | |
| Host / Isotype: | Mouse / IgG |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 42C617.1.2 |
| Species Reactivity: | Human (Hu) Murine (Ms) |
| Immunogen: | Human DAP-3 protein. |
| Ordering Information | ||||
| Pierce Anti-DAP3 Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-41279 | 100 µg | $285.00 | ||
| Storage: | -20°C |
| Form: | 100 µg antibody in 200 µl PBS containing 0.02% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | 1-2 µg/ml |
| Product Specific Information |
| MA1-41279 detects DAP3 in murine and human samples. MA1-41279 has been successfully used in Western blot applications. MA1-41279 is derived from a synthetic peptide corresponding to amino acids 51-66 of human DaP-3 protein. This sequence is 75% homologous in mouse and rat. |
| General Information |
| Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (3). |
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Western Blot with anti-DAP3 Monoclonal Antibody [42C617.1.2] (MA1-41279)
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(This product is for In Vitro experimental use only.)
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PO
Box 117, Rockford, IL 61105 USA |
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