| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | WM59 |
| Species Reactivity: | Human (Hu) Non-human primate (Nhp) |
| Immunogen: | Human CD31 |
| Ordering Information | ||||
| Pierce Anti-CD31 Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-26196 | 100 µg | $356.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of protein G purified antibody (1 mg/ml) in PBS with 0.09% sodium azide; pH 7.4. |
| Applications | Dilution |
| Flow Cytometry (FACS) | 1:50-1:100 |
| Immunocytochemistry (ICC) | 1:50 |
| Immunoprecipitation (IP) | Assay Dependent |
| Product Specific Information |
| MA1-26196 detects CD31 from human, Cynomolgus monkey, and Rhesus monkey samples. MA1-26196 has been successfully used in flow cytometry, immunocytochemistry and immunoprecipitation procedures. In FACS use 10 µl of the suggested working dilution to label 10^6 cels in 100 µl. Figure 1 illustrates immunofluorescent staining of CD31 (red) in rat vascular endothelial cells using MA1-26196. Nuclear DAPI (blue) and FAAH (green) staining are also shown. |
| General Information |
| CD31 antibodies are a superior marker for angiogenesis, when compared to Factor VIII and CD34 antibodies. CD31 has been used to measure angiogenesis in association with tumor recurrence. CD31 used in a panel with CD34 and Factor VIII has also been used to mark Kaposi's sarcoma and angiosarcomas. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). |
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