Anti-CHOP Monoclonal Antibody
| Host / Isotype: |
Mouse / IgG2b |
| Class: |
Monoclonal |
| Type: |
Antibody |
| Clone: |
9C8 |
| Label: |
|
| Species Reactivity: |
Human (Hu) Murine (Ms) |
| Immunogen: |
A bacterially expressed, mouse CHOP fusion protein. |
|
| Immunoprecipitation (IP) |
Assay dependent |
| Western Blot (WB) |
1 ug/ml |
| Pierce Anti-CHOP Monoclonal Antibody |
|
| Product Number |
Pkg Size |
Price |
|
|
| MA1-250 |
100 µg |
$354.00 |
|
|
|
|
| Storage: |
-20º C, Avoid Freeze/Thaw Cycles |
| Form: |
100 µg of purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
MA1-250 detects CHOP protein in human and mouse samples.
MA1-250 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract.
The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP.
Figure 1 shows a Western blot of endogenous CHOP from primary human fibroblasts using MA1-250.
Lane 1: Untreated cells
Lane 2: Cells treated with tunicamycin for 10 hours |
CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets.
Under normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. |
|
| J. Cell Sci., 110: 1741-1750, 1997 |
| EMBO, 17(13): 3619-3630, 1998 |
| EMBO, 14(19): 4654-4661, 1995 |
| PNAS, 96: 5025-5030, 1999 |
| EMBO 17(19): 5708-5717, 1998 |
| Mol. Cell. Bio., 19(1): 495-504, 1999 |
This product is for In Vitro experimental use only
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