Anti-CHOP Monoclonal Antibody
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Synonyms:
GADD153, Growth Arrest and DNA Damage Inducible Protein 153, C/EBP Homology Protein
Entrez Gene ID:
(Human) 1649, (Murine) 13198
UniProt ID:
P35638
| Details | |
| Host / Isotype: | Mouse / IgG2b |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 9C8 |
| Species Reactivity: | Human (Hu) Murine (Ms) |
| Immunogen: | A bacterially expressed, mouse CHOP fusion protein. |
| Ordering Information | ||||
| Pierce Anti-CHOP Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-250 | 100 µg | $354.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | 1 ug/ml |
| Product Specific Information |
| MA1-250 detects CHOP protein in human and mouse samples. MA1-250 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract. The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP. Figure 1 shows a Western blot of endogenous CHOP from primary human fibroblasts using MA1-250. Lane 1: Untreated cells Lane 2: Cells treated with tunicamycin for 10 hours |
| General Information |
| CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. |
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Western Blot with anti-CHOP Monoclonal Antibody [9C8] (MA1-250)
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| References: |
| J. Cell Sci., 110: 1741-1750, 1997 |
| PNAS, 96: 5025-5030, 1999 |
| EMBO 17(19): 5708-5717, 1998 |
| EMBO, 14(19): 4654-4661, 1995 |
| EMBO, 17(13): 3619-3630, 1998 |
| Mol. Cell. Bio., 19(1): 495-504, 1999 |
(This product is for In Vitro experimental use only.)
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