Part of Thermo Fisher Scientific

Anti-CHOP Monoclonal Antibody

Image Preview (1 image)
Synonyms:
GADD153, Growth Arrest and DNA Damage Inducible Protein 153, C/EBP Homology Protein
Entrez Gene ID:
(Human) 1649, (Murine) 13198
UniProt ID:
P35638
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 9C8
Species Reactivity: Human (Hu) Murine (Ms)
Immunogen: A bacterially expressed, mouse CHOP fusion protein.
Ordering Information
Pierce Anti-CHOP Monoclonal Antibody
Product Number Pkg. Size Price Purchase
MA1-250 100 µg $354.00


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µg of purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1 ug/ml
Product Specific Information
MA1-250 detects CHOP protein in human and mouse samples.

MA1-250 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract.

The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP.

Figure 1 shows a Western blot of endogenous CHOP from primary human fibroblasts using MA1-250.
Lane 1: Untreated cells
Lane 2: Cells treated with tunicamycin for 10 hours
General Information
CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets.
Under normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
Western Blot with anti-CHOP Monoclonal Antibody [9C8] (MA1-250)
References:
J. Cell Sci., 110: 1741-1750, 1997
PNAS, 96: 5025-5030, 1999
EMBO 17(19): 5708-5717, 1998
EMBO, 14(19): 4654-4661, 1995
EMBO, 17(13): 3619-3630, 1998
Mol. Cell. Bio., 19(1): 495-504, 1999
(This product is for In Vitro experimental use only.)


Learn more about other Thermo Scientific Life Science Research Products
Protein Research Products Cell Culture High Content Screening
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies European Sales

© 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723 or 815-968-0747

Trademarks Privacy Statement