Anti-ABCA1 Monoclonal Antibody
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Synonyms:
ABC 1 antibody, ABC Transporter 1 antibody, ABC1 antibody, ATP-binding Cassette 1 antibody, ATP-binding Cassette Transporter 1 antibody, CERP antibody
Entrez Gene ID:
(Murine) 11303
UniProt ID:
P41233
| Details | |
| Host / Isotype: | Rat / IgG1, kappa |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 3A1.891.3 |
| Species Reactivity: | Murine (Ms) |
| Immunogen: | Synthetic peptide corrseponding to the N-terminal loop residues 200-250 of mouse ABCA1. |
| Ordering Information | ||||
| Pierce Anti-ABCA1 Monoclonal Antibody | ||||
| Product Number | Pkg. Size | Price | Purchase | |
| MA1-16936 | 100 µl | $340.00 | ||
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of diluted ascites containing 0.1% sodium azide. |
| Applications | Dilution |
| Western Blot (WB) | 1:1000 |
| Product Specific Information |
| MA1-16936 detects ABCA1 protein in mouse samples. This antibody does not react with human ABCA1. MA1-16936 has been successfully used in Western blot procedures. By Western blot, MA1-16936 is specific for the ~250 kDa ABCA1 protein. Important note: ABCA1 is known to aggregate. DO NOT BOIL SAMPLES. Use a reducing buffer: 2X sample buffer: 8M Urea, 2% SDS, 715 mM BME, 1% glycerol, 0.001% Bromophenol Blue, 125 mM Tris-HCl [pH 6.8]. The MA1-16936 immunogen is a synthetic peptide corrseponding to the N-terminal loop residues 200-250 of mouse ABCA1. Figure 1 shows a Western blot detection of ABCA1 using MA1-16936 at various dilutions in ABCA1 transfected HeLa lysates. |
| General Information |
| Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI. |
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Western Blot with anti-ABCA1 Monoclonal Antibody [3A1.891.3] (MA1-16936)
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(This product is for In Vitro experimental use only.)
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PO
Box 117, Rockford, IL 61105 USA |
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